Abstract

Review Article

Free Radicals, Antioxidants and Redox Potential

Richard Rokyta* and Holeček V

Published: 29 May, 2025 | Volume 9 - Issue 1 | Pages: 005-010

The role of free radicals and antioxidants is often underestimated despite their involvement in key metabolic processes, although they participate in many important metabolic processes in the life of humans, animals and plants. Their quantity and quality differ from each other, which is not respected. Each cell is attacked approximately 10,000 times by free radicals. Oxidative stress is the cause of many problems, especially in viral diseases. Monitoring of redox potentials in body fluids is usually not carried out. Viral replication is influenced by oxidative energy, derived from either host metabolism or free radical activity, which is supplied by oxidation by free radicals or the host. Nucleic acid mutations due to the effect of free radicals can be the cause of carcinomas, and possible defense against mutations could help eradicate dangerous viruses. The importance of malondialdehyde and antibodies against it is discussed. Eliminating free radicals, reducing lipoperoxidation, and protecting against environmental oxidative stress are important factors for human health.
Study objective: To highlight the importance of free radicals, antioxidants and redox potentials for patient diagnosis and therapy.
Methods: This study synthesizes findings from multiple published sources, including our own research.
Results: Findings highlight the role of free radicals in oxidative stress, DNA damage, and viral replication, with redox potential (ORP) and Malondialdehyde (MDA) identified as key diagnostic markers.
Conclusion: Monitoring oxidative balance and targeting free radical activity are essential for preventing cellular damage and improving clinical outcomes in oxidative stress-related diseases.

Read Full Article HTML DOI: 10.29328/journal.hor.1001031 Cite this Article Read Full Article PDF

Keywords:

Free radicals; Antioxidants; Mutations; Diseases; Oxidative stress; Aging; Malondialdehyde; Oxido-reductive potentials

References

  1. Muralidharan A, Bauer C, Katafiasz DM, Oyewole OO, Morwitzer MJ, Roy E, et al. Malondialdehyde acetaldehyde adduction of surfactant protein D attenuates SARS-CoV-2 spike protein binding and virus neutralization. Alcohol Clin Exp Res. 2023;47(1):95–103. Available from: https://doi.org/10.1111/acer.14974
  2. Martín-Fernández M, Aller R, Heredia-Rodríguez M, Gómez-Sánchez E, Martínez-Paz P, Gonzalo-Benito H, et al. Lipid peroxidation as a hallmark of severity in COVID-19 patients. Redox Biol. 2021;48:102181. Available from: https://doi.org/10.1016/j.redox.2021.102181
  3. Pláteník J. Free radicals, antioxidants and aging. Intern Med (Czech Republic). 2009;11(1):30–3. Available from: https://www.internimedicina.cz/pdfs/int/2009/01/06.pdf
  4. Nair CL, O'Neil P, Wang G. Malondialdehyde. In: Encyclopedia of Reagents for Organic Synthesis. New York: John Wiley & Sons; 2008. Available from: https://doi.org/10.1002/047084289X.rm013.pub2
  5. Ebrahimi M, Norouzi P, Aazami H, Moosavi-Movahedi AA. Review on oxidative stress relation on COVID-19: Biomolecular and bioanalytical approach. Int J Biol Macromol. 2021;189:802–18. Available from: https://doi.org/10.1016/j.ijbiomac.2021.08.095
  6. Hiffler L, Rakotoambinina B. Selenium deficiency promotes mutations, replication, and virulence of RNA viruses, and selenium has clinical benefit in RNA viral infections. Front Nutr. 2020;7:164. Available from: https://doi.org/10.3389/fnut.2020.00164
  7. Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR. Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage. Nature. 2017 Nov 23;551(7681):464-471. Available from:https://doi.org/10.1038/nature24644
  8. Saso L, Orhan HG, Stepanić V. Modulators of oxidative stress: chemical and pharmacological aspects. Antioxidants (Basel). 2020;9(8):657. Available from: https://doi.org/10.3390/antiox9080657
  9. Svoboda T. 2020 Grothman statement on fenofibrate’s effect on COVID-19 [Internet]. Glenbeulah (WI): Congressman Glenn Grothman; 2020. Available from: https://grothman.house.gov/news/documentsingle.aspx?DocumentID=1669
  10. Kalousová M. Patobiochemie ve schématech. 1st ed. Praha: Grada; 2006.
  11. Zheng Y, Zhu D. Molecular hydrogen therapy ameliorates organ damage induced by sepsis. Oxid Med Cell Longev. 2016;2016:5806057. Available from: https://doi.org/10.1155/2016/5806057
  12. Muhammad Y, Kani YA, Iliya S, Muhammad JB, Binji A, El-Fulaty AA, et al. Deficiency of antioxidants and increased oxidative stress in COVID-19 patients: A cross-sectional comparative study in Jigawa, Northwestern Nigeria. SAGE Open Med. 2021;9:2050312121991246. Available from: https://doi.org/10.1177/2050312121991246
  13. Slezák J, Kura B, Frimmel K, Zálešák M, Ravingerová T, Viczenczová C, et al. Preventive and therapeutic application of molecular hydrogen in situations with excessive production of free radicals. Physiol Res. 2016;65 Suppl 1:S11–28. Available from: https://doi.org/10.33549/physiolres.933414
  14. Dasuri K, Zhang L, Keller JN. Oxidative stress, neurodegeneration, and the balance of degradation and protein synthesis. Free Radic Biol Med. 2013;62:170–85. Available from: http://dx.doi.org/10.1016/j.freeradbiomed.2012.09.016
  15. Ameer K. Avocado as a major dietary source of antioxidants and its preventive role in neurodegenerative diseases. Adv Neurobiol. 2016;12:337–54. Available from: https://doi.org/10.1007/978-3-319-28383-8_18
  16. Chmátalová Z, Skoumalová A. Oxidative stress in Alzheimer's disease and its consequences. Klin Biochem Metab. 2014;43(4):189–95. Available from: https://casopiskbm.cz/pdfs/kbm/2014/04/04.pdf
  17. Veech RL. The determination of the redox state and phosphorylation potential in living tissues and relationship to metabolic control of disease phenotype. Biochemistry and Metabolic Biology. 2006;34(3):168–79. Available from: https://doi.org/10.1002/bmb.2006.49403403168
  18. Gilgun-Sherki Y, Melamed E, Offen D. Oxidative stress induced-neurodegenerative diseases: the need for antioxidants that penetrate the blood brain barrier. Neuropharmacology. 2001;40(8):959–75. Available from: https://doi.org/10.1016/s0028-3908(01)00019-3
  19. Babbs CF. Free radicals and the etiology of colon cancer. Free Radic Biol Med. 1990;8(2):191–200. Available from: https://doi.org/10.1016/0891-5849(90)90091-v
  20. Holecek V, Kulich V. [Influence of human erythrocytes on the synthesis of nicotinamide mononucleotide in vitro]. Clin Chim Acta. 1962;7:652–6. Available from: https://doi.org/10.1016/0009-8981(62)90146-8
  21. Lang CA, Mills BJ, Lang HL, Liu MC, Usui WM, Richie JP Jr, Mastropaolo W, Murrell SA. High blood glutathione levels accompany excellent physical and mental health in women ages 60 to 103 years. J Lab Clin Med. 2002;140(6):413–7. Available from: https://doi.org/10.1067/mlc.2002.129504
  22. Ortolani O, Conti A, De Gaudio AR, Moraldi E, Novelli GP. [Glutathione and N-acetylcysteine in the prevention of free-radical damage in the initial phase of septic shock. Recenti Prog Med. 2002;93(2):125–9. Available from: https://pubmed.ncbi.nlm.nih.gov/11887346/

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